Luke H. Bradley

  • Associate Professor
  • Neuroscience
MN 214 Medical Sciences Bldg
859-323-1826
Other Affiliations:
  • Anatomy & Neurobiology
  • Molecular & Cellular Biochemistry
Bio / Education
BS, Chemistry/Biochemistry, Ohio University Ph.D., Biochemistry, Ohio State University Postdoctoral Training, Chemistry/Synthetic Biology, Princeton University
Research Description
Peptide and protein scaffolds for biotherapeutic discovery and development Research in the Bradley laboratory utilizes approaches at the interface of chemistry, biochemistry, and neurobiology to discover and develop peptide and protein-based molecules as platforms for various medical, biotechnical, and biotherapeutic applications. We are using molecular design and screening techniques to develop molecules with desired biological activities and properties. In addition, biochemical and neurobiological characterization will contribute towards understanding the basis of the native molecule's function.
Selected Publications
Fuqua JL, Littrell OM, Lundblad M, Turchan-Cholewo J, Abdelmoti LG, Galperin E, Bradley LH, Cass WA, Gash DM, Gerhardt GA. (2014) Dynamic Changes in the Dopamine Neuron Function after DNSP-11 Treatment: Effects in vivo and Increased ERK 1/2 Phosphorylation in vitro. Peptides 54: 1-8. Hardy PA, Keeley D, Schorn G, Forman, E, Ai Y, Zhang Z, Bradley LH. (2013) Convection enhanced delivery of gadolinium-tagged polylysine. J Neuroscience Methods, 219: 169-175. Littrell OM, Fuqua JF, Richardson AD, Turchan-Cholewo J, Hascup ER, Huettl P, Pomerleau F, Bradley LH, Gash DM, Gerhardt GA. (2013) A synthetic five amino acid GDNF propeptide increases dopamine neuron differentiation and neurochemical function. Neuropeptides, 47: 43-49. Magnani R, Chaffin B, Dick E, Bricken ML, Houtz RL, Bradley LH. (2012) Utilization of a calmodulin lysine methyltransferase co-expression system for the generation of a combinatorial library of post-translationally modified proteins. Protein Expression & Purification, 86: 83-88. Sexton T, Boock LJ, Rodgers DR, Bradley LH, Hersh LB. (2012) Active site mutations change the substrate cleavage specificity of neprilysin. PLoS ONE, 7(2): e32343. Arai R, Kobayashi N, Kimura A, Sato T, Wang AF, Platt JM, Bradley LH, Hecht MH. (2012) Domain-swapped dimeric structure of a de novo 4-helix bundle protein, WA20. J Phys Chem B, 116: 6569-7032. Bradley LH, Bricken ML, Randle C. (2011) Expression, purification, and characterization of proteins from high-quality combinatorial libraries of the mammalian calmodulin central linker. Protein Expression & Purification 75: 186-192. Kelps KA, Turchan-Cholewo J, Hascup ER, Taylor TL, Gash DM, Gerhardt GA, Bradley LH. (2011) Evaluation of the physical and in vitro protective activity of three synthetic peptides derived from the pro- and mature GDNF sequence. Neuropeptides 45(3): 213-218. Bradley LH, Fuqua J, Richardson A, Turchan-Cholewo J, Ai Y, Kelps KA, Glass J, He X, Zhang Z, Grondin R, Littrell OM, Gash DM, Gerhardt GA. (2010) Dopamine neuron stimulating actions of a GDNF propeptide. PLoS ONE 5(3): e9752. Patel SC, Bradley LH, Jinadasa SP, Hecht MH. (2009) Cofactor Binding and Enzymatic Activity in an Unevolved Superfamily of De Novo Designed 4-Helix Bundle Proteins. Protein Science, 18:1388-1400. Bradley LH, Thumfort PP, Hecht MH. (2006) De novo proteins from binary patterned combinatorial libraries. Methods Mol Biol 340: 53-69. Bradley LH, Kleiner RE, Wang AF, Hecht MH, Wood DW. (2005) An Intein-Based Genetic Selection Enables Construction of a High-Quality Library of Binary Patterned De Novo Protein Sequences. Protein Engineering, Design & Selection 18: 201-207.
PubMed Publications*: 
  • Grondin, R ;Littrell, OM ;Zhang, Z.;Ai, Y ;Huettl, P.;Pomerleau, F ;Quintero, JE ;Andersen, AH ;Stenslik, MJ ;Bradley, LH ;Lemmon, J ;O'Neill, MJ ;Gash, DM ;Gerhardt, GA "GDNF revisited: A novel mammalian cell-derived variant form of GDNF increases dopamine turnover and improves brain biodistribution." Neuropharmacology (2018): Details. Full text
  • Stenslik, MJ ;Evans, A ;Pomerleau, F ;Weeks, R ;Huettl, P.;Foreman, E ;Turchan-Cholewo, J ;Andersen, A ;Cass, WA ;Zhang, Z.;Grondin, RC ;Gash, DM ;Gerhardt, GA ;Bradley, LH "Methodology and effects of repeated intranasal delivery of DNSP-11 in awake Rhesus macaques." Journal of neuroscience methods 303, (2018): 30-40. Details. Full text
  • Stenslik, MJ ;Potts, LF ;Sonne, JW ;Cass, WA ;Turchan-Cholewo, J ;Pomerleau, F ;Huettl, P.;Ai, Y ;Gash, DM ;Gerhardt, GA ;Bradley, LH "Methodology and effects of repeated intranasal delivery of DNSP-11 in a rat model of Parkinson's disease." Journal of neuroscience methods 251, (2015): 120-9. Details. Full text
  • Fuqua, JL ;Littrell, OM ;Lundblad, M ;Turchan-Cholewo, J ;Abdelmoti, LG ;Galperin, E ;Bradley, LH ;Cass, WA ;Gash, DM ;Gerhardt, GA "Dynamic changes in dopamine neuron function after DNSP-11 treatment: effects in vivo and increased ERK 1/2 phosphorylation in vitro." Peptides 54, (2014): 1-8. Details. Full text
  • Littrell, OM ;Fuqua, JL ;Richardson, AD ;Turchan-Cholewo, J ;Hascup, ER ;Huettl, P.;Pomerleau, F ;Bradley, LH ;Gash, DM ;Gerhardt, GA "A synthetic five amino acid propeptide increases dopamine neuron differentiation and neurochemical function." Neuropeptides 47, 1 (2013): 43-9. Details. Full text
  • Hardy, PA ;Keeley, D ;Schorn, G ;Forman, E ;Ai, Y ;Venugopalan, R ;Zhang, Z.;Bradley, LH "Convection enhanced delivery of different molecular weight tracers of gadolinium-tagged polylysine." Journal of neuroscience methods 219, 1 (2013): 169-75. Details. Full text
  • Magnani, R ;Chaffin, B ;Dick, E ;Bricken, ML ;Houtz, RL ;Bradley, LH "Utilization of a calmodulin lysine methyltransferase co-expression system for the generation of a combinatorial library of post-translationally modified proteins." Protein expression and purification 86, 2 (2012): 83-8. Details. Full text
  • Arai, R ;Kobayashi, N ;Kimura, A ;Sato, T ;Matsuo, K ;Wang, AF ;Platt, JM ;Bradley, LH ;Hecht, MH "Domain-swapped dimeric structure of a stable and functional de novo four-helix bundle protein, WA20." The journal of physical chemistry. B 116, 23 (2012): 6789-97. Details. Full text
  • Bradley, LH ;Bricken, ML ;Randle, C "Expression, purification, and characterization of proteins from high-quality combinatorial libraries of the mammalian calmodulin central linker." Protein expression and purification 75, 2 (2011): 186-91. Details. Full text
  • Fisher, MA ;McKinley, KL ;Bradley, LH ;, ;Hecht, MH "De novo designed proteins from a library of artificial sequences function in Escherichia coli and enable cell growth." PloS one 6, 1 (2011): e15364. Details. Full text
  • Kelps, KA ;Turchan-Cholewo, J ;Hascup, ER ;Taylor, TL ;Gash, DM ;Gerhardt, GA ;Bradley, LH "Evaluation of the physical and in vitro protective activity of three synthetic peptides derived from the pro- and mature GDNF sequence." Neuropeptides 45, 3 (2011): 213-8. Details. Full text
  • Bradley, LH ;Fuqua, J ;Richardson, A.;Turchan-Cholewo, J ;Ai, Y ;Kelps, KA ;Glass, JD ;He, X ;Zhang, Z.;Grondin, R ;Littrell, OM ;Huettl, P.;Pomerleau, F ;Gash, DM ;Gerhardt, GA "Dopamine neuron stimulating actions of a GDNF propeptide." PloS one 5, 3 (2010): e9752. Details. Full text
  • Patel, SC ;Bradley, LH ;Jinadasa, SP ;Hecht, MH "Cofactor binding and enzymatic activity in an unevolved superfamily of de novo designed 4-helix bundle proteins." Protein science : a publication of the Protein Society 18, 7 (2009): 1388-400. Details. Full text
  • Bradley, LH ;Paullus, WC ;Howe, J ;Litofsky, NS "Isolated transverse process fractures: spine service management not needed." The Journal of trauma 65, 4 (2008): 832-6; discussion 836. Details. Full text
  • Bradley, LH ;Kleiner, RE ;Wang, AF ;Hecht, MH ;Wood, DW "An intein-based genetic selection allows the construction of a high-quality library of binary patterned de novo protein sequences." Protein engineering, design & selection : PEDS 18, 4 (2005): 201-7. Details. Full text
  • Hecht, MH ;Das, A ;Go, A ;Bradley, LH ;Wei, Y "De novo proteins from designed combinatorial libraries." Protein science : a publication of the Protein Society 13, 7 (2004): 1711-23. Details. Full text
  • Chang, F ;Bradley, LH ;Swenson, RP "Evaluation of the hydrogen bonding interactions and their effects on the oxidation-reduction potentials for the riboflavin complex of the Desulfovibrio vulgaris flavodoxin." Biochimica et biophysica acta 1504, 2-3 (2001): 319-28. Details. Full text
  • Bradley, LH ;Swenson, RP "Role of hydrogen bonding interactions to N(3)H of the flavin mononucleotide cofactor in the modulation of the redox potentials of the Clostridium beijerinckii flavodoxin." Biochemistry 40, 30 (2001): 8686-95. Details. Full text
  • Bradley, LH ;Swenson, RP "Role of glutamate-59 hydrogen bonded to N(3)H of the flavin mononucleotide cofactor in the modulation of the redox potentials of the Clostridium beijerinckii flavodoxin. Glutamate-59 is not responsible for the pH dependency but contributes to the..." Biochemistry 38, 38 (1999): 12377-86. Details. Full text
  • Bradley, LH ;Thumfort, PP ;Hecht, MH "De novo proteins from binary-patterned combinatorial libraries." Methods in molecular biology (Clifton, N.J.) 340, (0): 53-69. Details. Full text
  • Bradley, LH "High-quality combinatorial protein libraries using the binary patterning approach." Methods in molecular biology (Clifton, N.J.) 1216, (0): 117-28. Details. Full text
  • Sexton, T ;Hitchcook, LJ ;Rodgers, DW ;Bradley, LH ;Hersh, LB "Active site mutations change the cleavage specificity of neprilysin." PloS one 7, 2 (0): e32343. Details. Full text
  • Bradley, LH ;Wei, Y ;Thumfort, P ;Wurth, C ;Hecht, MH "Protein design by binary patterning of polar and nonpolar amino acids." Methods in molecular biology (Clifton, N.J.) 352, (0): 155-66. Details. Full text
  • Lim, RG ;Rawlings, AL ;Schultz, SL ;Bradley, LH ;Hammad, HT ;Bechtold, ML "Unusual cause of constipation: sunflower seeds bezoar." Endoscopy 45 Suppl 2 UCTN, (0): E46. Details. Full text
  • Bradley, LH ;Burton, M ;Gokden, M ;Serletis, D "Prostate carcinoma mimicking a sphenoid wing meningioma." International journal of surgery case reports 15, (0): 63-5. Details. Full text
* Publications are automatically pulled from pubmed.gov based on a user-specific query. Results may include incorrect citations. See: Tutorial on improving PubMed results.
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