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Spring 2023

Mon, 01/09/2023 - 00:00

Mon, 05/01/2023 - 00:00

Res Exp Bio: Anls Gene Exp Slmdr Tl Rege

BIO 199 006: Analysis of Gene Expression During Salamander Tail Regeneration Salamanders are remarkable in their ability to regenerate damaged or missing body parts, including limbs and tail. Students will perform tail amputation surgeries on axolotl embryos and then rear these in the presence or absence of a chemical that is known to inhibit the function of a specific molecular signaling pathway. After the class establishes that the chemical alters tail regeneration, the tail amputation experiment will be repeated and regenerating tissue collected for RNA isolation and gene expression analysis. The data from the gene expression analysis will be used to identify when and where the targeted molecular signaling pathway functions during regeneration, and to identify down-stream gene expression targets. Overall, the exercise will introduce students to experimental approaches and data analysis techniques that are currently being used to resolve the molecular basis of tissue regeneration. Controlled enrollment. No contract needed. Open only to STEMCats students until November 20, 2022. Honors College students may enroll beginning November 21, 2022 after obtaining permission from the course instructor to receive an override to register. Other students may enroll beginning November 28, 2022 after obtaining permission from the course instructor to receive an override to register.