By Whitney Harder
(April 25, 2016) — A new instrument to be developed by University of Kentucky researchers will overcome current limitations in fluorescence microscopy and could accelerate basic scientific discoveries. The multimodal and cost-effective imaging and data collection platform is being funded by a three-year, $589,250 National Science Foundation (NSF) grant from the Instrument Development for Biological Research (IDBR) program.
Total internal reflection fluorescence (TIRF) microscopy and fluorescence correlation spectroscopy (FCS) have become essential tools to understand biochemical and cellular processes. But these tools are limited — they are often highly labor intensive and thus have been primarily restricted to single sample analysis followed by costly manual data processing.