Judith Lesnaw

  • Professor Emeritus
(859) 257-4711
Education

Ph.D. Univ. of Illinois, Urbana 1969

Research

Molecular Virology

My research program is focused on understanding the mechanisms of negative-stand RNA virus transcription and replication. Our model system is the transcription complex of vesicular stomatitis virus (VSV) which contains a large multifunctional protein. We are integrating genetic, biochemical and recombinant DNA technologies to the identification of functional domains within the VSV transcription complex. Specific approaches include: identification of functional and structural mutational lesions in spontaneous mutants; generation of site-directed mutations targeted to conserve regions which we recently identified by direct RNA sequencing; and enzyme active site-directed photoaffinity labeling. Knowledge that emerges from these studies will direct the design of new therapeutic agents for a wide array of human pathogens, and will contribute to our understanding of proteins that contain useful constellations of enzyme activities.

Selected Publications: 
  • Hammond, David C., Robert K. Evans, and Judith A. Lesnaw (1992). The L Protein of Vesicular Stomatitis Virus Transcription Complexes is Specifically Photolabeled by 5-Azido-uridine 5'-Triphosphate, an Analog of the RNA Polymerase Substrate Uridine 5'-Triphosphate. J. Gen. Virol. 73:61-66.
  • Hammond, David C., Boyd E. Haley, and Judith A. Lesnaw (1992). Identification and Characterization of Serine/Threonine Protein Kinase Activity Intrinisic to the L Protein of VSV New Jersey. J. Gen Virol. 73:67-75.
  • van den Heuvel, J.F.J.M., D.W. Thornbury, T.P. Pirone, and J.A. Lesnaw (1993). A simple and efficient procedure for the oral inoculation of Trichoplusia ni larvae with polyhedrin-negative recombinant baculovirus. J. Virol. Methods 42:207-216.
PubMed Publications*: 
  • Ghosh, D.;Brooks, RE ;Wang, R.;Lesnaw, J.;Goodin, MM "Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus." Virus research 135, 1 (2008): 26-35. Details. Full text
  • Goodin, M.;Yelton, S.;Ghosh, D.;Mathews, S.;Lesnaw, J. "Live-cell imaging of rhabdovirus-induced morphological changes in plant nuclear membranes." Molecular plant-microbe interactions : MPMI 18, 7 (2005): 703-9. Details. Full text
  • Evans, ME ;Lesnaw, JA "Infection control for gene therapy: a busy physician's primer." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 35, 5 (2002): 597-605. Details. Full text
  • Lesnaw, JA ;Ghabrial, SA "Tulip Breaking: Past, Present, and Future." Plant disease 84, 10 (2000): 1052-1060. Details. Full text
  • Evans, ME ;Lesnaw, JA "Infection control in gene therapy." Infection control and hospital epidemiology 20, 8 (1999): 568-76. Details. Full text
  • Thornbury, DW ;Heuvel, JF ;Lesnaw, JA ;Pirone, TP "Expression of potyvirus proteins in insect cells infected with a recombinant baculovirus." The Journal of general virology 74 ( Pt 12), (1993): 2731-5. Details. Full text
  • Heuvel, JF ;Thornbury, DW ;Pirone, TP ;Lesnaw, JA "A simple and efficient procedure for the oral inoculation of Trichoplusia ni larvae with polyhedrin-negative recombinant baculovirus." Journal of virological methods 42, 2-3 (1993): 207-15. Details. Full text
  • Hammond, DC ;Haley, BE ;Lesnaw, JA "Identification and characterization of serine/threonine protein kinase activity intrinsic to the L protein of vesicular stomatitis virus New Jersey." The Journal of general virology 73 ( Pt 1), (1992): 67-75. Details. Full text
  • Hammond, DC ;Evans, RK ;Lesnaw, JA "The L protein of vesicular stomatitis virus transcription complexes is specifically photolabelled by 5-azido-uridine 5'-triphosphate, an analogue of the RNA polymerase substrate uridine 5'-triphosphate." The Journal of general virology 73 ( Pt 1), (1992): 61-6. Details. Full text
  • Feldhaus, AL ;Lesnaw, JA "Nucleotide sequence of the L gene of vesicular stomatitis virus (New Jersey): identification of conserved domains in the New Jersey and Indiana L proteins." Virology 163, 2 (1988): 359-68. Details.
  • Hammond, DC ;Lesnaw, JA "Functional analysis of hypomethylation variants of the New Jersey serotype of vesicular stomatitis virus." Virology 160, 2 (1987): 330-5. Details.
  • Hellmann, GM ;Shaw, JG ;Lesnaw, JA ;Chu, LY ;Pirone, TP ;Rhoads, RE "Cell-free translation of tobacco vein mottling virus RNA." Virology 106, 2 (1980): 207-16. Details. Full text
* Publications are automatically pulled from pubmed.gov based on a user-specific query. Results may include incorrect citations. See: Tutorial on improving PubMed results.
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