Mass spectrometry research in my group is divided into three broad areas: 1) proteomics and biological mass spectrometry, 2) environmental methods development working at the interface between environmental chemistry and biology and 3) quadrupole ion trap instrument development.

1. Proteomics projects include development of biomarkers in CSF of Alzheimer's Disease (AD), and neuronal mitochondrial changes in AD, transcription of MnSOD during tumorgenesis and determination of protein modifications in thermophilic anaerobic bacteria adapted to high ethanol environments.
2. Environmental methods development is currently focused on chemical alterations to proteins resulting from oxidative stress such as acrolein modification. We continue to be interested in pesticide toxicology and have recently focused on phosphorylation of the enzyme butyryl cholinesterase by organophosphorus insecticides. Example spectra: MALDI spectrum of the active site peptide, MALDI spectrum of the inhibited active site peptide, Capillary HPLC chromatogram of the inhibited active site peptide, and MS/MS spectrum of the inhibited active site peptide
3. A variety of quadrupole ion trap (QIT) projects are underway including development of miniature cylindrical ion traps, ion tomography experiments, ion mobility in a QIT and alternative scan functions.

• M. A. Lovell, S. Xiong, W. R. Markesbery and B. C. Lynn, "Quantitative Proteomics Analysis of Mitochondria from Primary Neuron Cultures Treated With Amyloid Beta Peptide," Neurochemical Research, 30, 113-122 (2005).
• C. B. Pocernich, D. B. Boyd-Kimball, H. F. Poon, V. Thongboonkerd, B.C. Lynn, J. B. Klein, V. Calebrese, A. Nath, D. A. Buttertfield, " Proteomics Analysis of Human Astrocytes expressing the HIV protein TAT," Mol. Brain Res., 133, 307-316 (2005).
• C. B. Pocernich, H. F. Poon, D. B. Boyd-Kimball, B.C. Lynn, A. Nath, J. B. Klein, D. A. Buttertfield, "Proteomic Analysis of Oxidatively Modified Proteins Induced by the Mitochondrial Toxin 3-nitropropionic acid in Human Astrocytes expressing the HIV protein TAT." Mol. Brain Res., 133, 299-306 (2005).
• D. B. Boyd-Kimball, R. Sultana, H. F. Poon, H. Mohmmad-Abdul, B.C. Lynn, J. B. Klein, D. A. Buttertfield, "g-Glutamylcysteine Ethyl Ester Protection of Proteins from Abeta(1-42)-Mediated Oxidative Stress in Neuronal Cell Culture: a Proteomics Approach." J. Neuroscience Res., 79, 707-713 (2005).
• H. F. Poon, S. A. Farr, V. Thongboonkerd, B.C. Lynn, W. A. Banks, J. E. Morley, J. B. Klein, D. A. Buttertfield, " Proteomic Analysis of Specific Brain Proteins in Aged SAMP8 Mice treated with alpha-lipoic acid: Implecations for Aging and Age-related Neurodegenerative Disorders." Neurochemistry International, 46, 159-168 (2005).
• T. I. Williams, M. A. Lovell, B.C. Lynn "Analysis of Derivatized Biogenic Aldehydes by HPLC Tandem Mass Spectrometry." Anal. Chem., 77, 3383-3389 (2005).
• X. Liu, M. A. Lovell, B. C. Lynn, “Development of an Isotope Dilution Capillary Liquid Chromatography Nanoelectrospray Tandem Mass Spectrometry Method for Detection and Quantification of Acrolein-Deoxyguanosine Adducts.” Anal. Chem. 77, 5982-5989 (2005).
• D. Boyd-Kimball, A. Castegna, R. Sultana, H.F. Poon, R. Petroze, B.C. Lynn, J.B. Klein, D.A. Butterfield, “Proteomic Identification of Proteins Specifically Oxidized by Abeta (1-42) in Synaptosomes: Implications for Alzheimer’s disease.” Brain Research, 1044, 206-215 (2005).
• Q. Wang, J. He, B.C. Lynn and B.C. Rymond, “Interactions of the yeast SF3b splicing factor” Mol. Cell. Biol., 25, 10745-10754 (2005).
• T. I. Williams, B. C. Lynn, W. R. Markesbery and M. A. Lovell, “Elevated 4-hydroxynonenal and Acrolein in Mild Cognitive Impairment and Early Alzheimer’s Disease.”, Neurobiol. Aging, 27, 1094-1099, (2006).
• T.I. Williams, J.C. Combs, A.P. Thakur, H.J. Strobel and B.C. Lynn “A Novel Bicine Running Buffer System for Doubled Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis of Membrane Proteins” Electrophoresis, 27, 2984-2995 (2006).
• X. Liu, M.A. Lovell and B.C. Lynn, “Detection and Quantification of Endogenous Cyclic DNA Adducts Derived from 4-hydroxynonenal in Human Brain Tissue by Isotope Dilution Capillary Liquid Chromatography Nanoelectrospray Tandem Mass Spectrometry” Chem. Res. Toxicol., 19, 710-718 (2006).
• S. Pandit, B. C. Lynn, B. C. Rymond, “Inhibition of a spliceosome turnover pathway suppresses splicing defects” Proc. Natl. Acad. Sci., 103, 13700-13705 (2006).
• A. T. McCollum, F. Jafarifar, B. C. Lynn, R. U. Agu, A. L. Stinchcomb, S. Wang, Q. Chen, R. P. Guttmann “Inhibition of Calpain-Mediated Cell Death by a Novel Peptide Inhibitor” Experimental Neurology,202, 506-513 (2006).
• T. I. Williams, J. C. Combs, B. C. Lynn, H. J. Strobel “Proteomic Profile Changes in Membranes of Ethanol-Tolerant Clostridium thermocellum” Appl. Microbiol. Biotechnol., 74, 422-432 (2007).
• A. M. Martin, T. Liu, B. C. Lynn, A. P. Sinai “The Toxoplasma gondii parasitophorous vacuole membrane: transactions across the border” Journal of Eukaryotic Microbiology, 54, 25-28 (2007).
• J. Sun. B.C. Lynn “Development of a MALDI-TOF-MS Method to Identify and Quantify Butyrylcholinesterase Inhibition Resulting from Exposure to Organophosphate and Carbamate Pesticides” J. Am. Soc. Mass Spectrom., 18, 698-706 (2007).
• A.M. Martin, T. Liu, B.C. Lynn, A.P. Sinai “A Elimination of Affinity Reagent Interference for the Mass Spectrometric Detection of Low-Abundance Proteins Following Immunoprecipitation” J. Proteome Res., 6, 4758-4762 (2007).
• Q. Wang, L. Zhang, B.C. Lynn and B.C. Rymond "A BBP–Mud2p heterodimer mediates branchpoint recognition and influences splicing substrate abundance in budding yeast"Nucleic Acids Research, 36, 2787-2798 (2008).